Abstract: A quantum memory, composed of a long-lived qubit coupled to each processing qubit, is important to building a scalable platform for quantum information science. These two qubits should be connected by a fast and high-fidelity operation to store and retrieve coherent quantum states. Here, we demonstrate a room-temperature quantum memory based on the spin of the nitrogen nucleus intrinsic to each nitrogen–vacancy (NV) centre in diamond. We perform coherent storage of a single NV centre electronic spin in a single nitrogen nuclear spin using Landau–Zener transitions across a hyperfine-mediated avoided level crossing. By working outside the asymptotic regime, we demonstrate coherent state transfer in as little as 120 ns with total storage fidelity of 88±6%. This work demonstrates the use of a quantum memory that is compatible with scaling as the nitrogen nucleus is deterministically present in each NV centre defect.